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1 \u5b9e\u9a8c\u90e8\u5206<\/p>\n

1.1 \u5b9e\u9a8c\u6750\u6599<\/p>\n

\u6740\u83cc\u5242\u6d3b\u6027\u7269\uff1a2-\u8f9b\u57fa-4-\u5f02\u567b\u5511\u5549-3-\u916e\uff08OIT\uff09,4\uff0c5-\u4e8c\u6c2f-N-\u8f9b\u57fa-4-\u5f02\u567b\u5511\u5549-3-\u916e(DCOIT),\u591a\u83cc\u7075(BCM) \u548c3-\u7898-2-\u4e19\u7094\u57fa\u4e01\u57fa\u6c28\u57fa\u7532\u9178\u4e01\u916f(IPBC)\uff1bGB\/T 1741\u20142007 \u76f8\u5173\u9709\u83cc\u3001ATCC6538 \u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\uff1b\u9a6c\u94c3\u85af\u8461\u8404\u7cd6\u743c\u8102\u57f9\u517b\u57fa(PDA)\uff1b\u6c34\u6027\u4e19\u70ef\u9178\u5f39\u6027\u5916\u5899\u6d82\u6599\uff1b\u6c34\u3001\u78f7\u9178\u76d0\u7f13\u51b2\u6db2\u3001\u7532\u9187\u3001\u4e59\u8148\u3001\u56db\u6c22\u544b\u5583\u3001\u4e59\u9178\u3002<\/p>\n

1.2 \u5b9e\u9a8c\u5668\u6750<\/p>\n

\u751f\u7269\u5b89\u5168\u67dc\uff0c\u57f9\u517b\u7bb1\uff0c\u6052\u6e29\u57f9\u517b\u6447\u5e8a\uff0c\u79fb\u6db2\u67aa\uff0c\u70d8\u7bb1\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff0c\u7d2b\u5916\u706f\u7bb1\uff0c\u663e\u5fae\u955c\uff0c\u8272\u5ea6\u4eea\uff0c\u6d82\u6599\u5e72\u819c\u51b2\u6c34\u88c5\u7f6e\uff0c\u51b0\u7bb1\uff0c\u8840\u7403\u8ba1\u6570\u677f\uff0c\u57f9\u517b\u76bf\uff0c\u63a5\u79cd\u73af\uff0c\u79bb\u5fc3\u7ba1\uff0c\u67aa\u5934\uff0c\u8fdb\u6837\u74f6\uff0c\u5236\u819c\u5668\uff0c\u5851\u6599\u819c\u7b49\u3002<\/p>\n

1.3 \u5b9e\u9a8c\u65b9\u6cd5<\/p>\n

1.3.1 \u5355\u72ec\u9632\u9709\u5242\u6d3b\u6027\u6210\u4efd\u7684\u9632\u9709\u6548\u679c\u6d4b\u8bd5<\/p>\n

\u5728\u7a7a\u767d\u6c34\u6027\u4e19\u70ef\u9178\u5f39\u6027\u5916\u5899\u6d82\u6599\u4e2d\u5206\u522b\u6dfb\u52a0100 \u00d710 -6\u3001200 \u00d710 -6\u3001300 \u00d710 -6\u3001400 \u00d710 -6\u3001500 \u00d710 -6 \u7684OIT\u3001DCOIT\u3001IPBC\u3001BCM\uff0c\u53c2\u8003GB\/T 1741\u20142007 \u65b9\u6cd5\uff0c\u4e3a\u5f3a\u5316\u5b9e\u9a8c\u82db\u523b\u6761\u4ef6\uff0c\u5c06\u57f9\u517b\u57fa\u4ece\u65e0\u673a\u76d0\u57f9\u517b\u57fa\u6362\u6210PDA\uff08\u9a6c\u94c3\u85af\u3001\u8461\u8404\u7cd6\u3001\u743c\u8102\uff09\u57f9\u517b\u57fa\u8fdb\u884c\u6d4b\u8bd5\uff0c \u56e0\u4e3aPDA \u57f9\u517b\u57fa\u8425\u517b\u66f4\u4e30\u5bcc\uff0c\u5fae\u751f\u7269\uff08\u9709\u83cc\uff09\u5728\u4e0a\u9762\u751f\u957f\u66f4\u65fa\u76db\uff0c\u8fd9\u6837\u5bf9\u9632\u9709\u5242\u7684\u6311\u6218\u6027\u5c31\u66f4\u5f3a\u3002<\/p>\n

\u5177\u4f53\u5b9e\u9a8c\u65b9\u6cd5\u4e3a\uff1a\u5c06\u6d82\u6599\u6837\u54c1\u5728\u7279\u79cd\u7eb8\u7247\u4e0a\u5236\u5907\u6210300 \u03bcm \u539a\u7684\u6e7f\u819c\u3001\u667e\u5e72\uff0c \u7136\u540e\u88c1\u526a\u621050 mm\u00d750mm \u7684\u6b63\u65b9\u5f62\u5757\uff0c \u63a5\u79cd\u9709\u83cc\u6df7\u5408\u5b62\u5b50\u60ac\u6d6e\u6db2\u5e76\u6d82\u5e03\u5747\u5300\uff0c\u4e4b\u540e\u5c06\u6837\u54c1\u5757\u653e\u7f6e\u4e8ePDA \u57f9\u517b\u57fa\u5e73\u677f\u4e2d\u95f4\u3002\u5c06\u5e73\u677f\u653e\u5165\u57f9\u517b\u7bb1\uff0c25 \u2103\u300185%\u76f8\u5bf9\u6e7f\u5ea6\u4e0b\u57f9\u517b4 \u5468\u3002\u6839\u636e\u6837\u54c1\u8868\u9762\u9709\u83cc\u751f\u957f\u60c5\u51b5\u8fdb\u884c\u8bc4\u5206\u3002\u63a5\u79cd\u9709\u83cc\u89c1\u88681\u3002<\/p>\n

1622112564477480.jpg<\/p>\n

1.3.2 4 \u79cd\u9632\u9709\u5242\u6d3b\u6027\u6210\u4efd\u7684\u8868\u9762\u6297\u83cc\u6548\u679c\u6d4b\u8bd5<\/p>\n

\u5728\u7a7a\u767d\u7684\u6c34\u6027\u5efa\u7b51\u6d82\u6599\u4e2d\u5206\u522b\u6dfb\u52a0500\u00d710-6OIT\u3001DCOIT\u3001IPBC\u3001BCM\uff0c \u53c2\u7167JIS Z 2801 \u65b9\u6cd5\uff0c \u4f7f\u7528ATCC6538 \u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u6d4b\u8bd54 \u79cd\u9632\u9709\u5242\u7684\u5e72\u819c\u8868\u9762\u6297\u83cc\u6548\u679c\u3002\u5177\u4f53\u5b9e\u9a8c\u65b9\u6cd5\u5982\u4e0b\uff1a\u5c06\u6d82\u6599\u6837\u54c1\u5728\u7279\u79cd\u7eb8\u7247\u4e0a\u5236\u5907\u6210300 \u03bcm \u539a\u7684\u6e7f\u819c\u3001\u667e\u5e72\uff0c \u7136\u540e\u88c1\u526a\u621050 mm\u00d750 mm \u7684\u6b63\u65b9\u5f62\u5757\u3002\u51c6\u5907ATCC6538 \u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u7684\u65b0\u9c9c\u83cc\u6db2\uff0c\u7a00\u91ca\u81f3\uff080.25\uff5e1\uff09\u00d7106 cfu\/mL,\u54380.4mL \u63a5\u79cd\u81f3\u5e72\u819c\u8868\u9762\u5e76\u76d6\u4e0a40 mm\u00d740 mm \u7684PE \u819c\u3002<\/p>\n

\u5c06\u5e73\u677f\u653e\u5165\u57f9\u517b\u7bb1\uff0c37 \u2103\u300190%\u76f8\u5bf9\u6e7f\u5ea6\u4e0b\u57f9\u517b24 h\u540e\uff0c\u901a\u8fc7\u68af\u5ea6\u7a00\u91ca\u6cd5\u8ba1\u6570\u83cc\u6b8b\u7559\u91cf\u3002<\/p>\n

1.3.3 4 \u79cd\u9632\u9709\u5242\u5728\u6d82\u6599\u4e2d\u7684\u70ed\u7a33\u5b9a\u6027\u6d4b\u8bd5<\/p>\n

\u5728\u6d82\u6599\u4e2d\u5206\u522b\u52a0\u5165\u7ea61 000\u00d710-6 \u7684OIT\u3001DCOIT\u3001IPBC\u3001BCM \u8fd94 \u79cd\u9632\u9709\u5242\uff0c\u5bc6\u5c01\u597d\u540e\u653e\u572855 \u2103\u70d8\u7bb1\u4e2d\u8001\u5316<\/a>4 \u5468\uff0c\u5206\u522b\u68c0\u6d4b\u8001\u5316\u524d\u540e\u6837\u54c1\u4e2dOIT\u3001DCOIT\u3001IPBC\u3001BCM \u7684\u542b\u91cf\u3002<\/p>\n

1.3.4 4 \u79cd\u9632\u9709\u5242\u6d3b\u6027\u6210\u4efd\u7684\u8010\u5019\u6027\u80fd\u6d4b\u8bd5<\/p>\n

\u5728\u7a7a\u767d\u7684\u6c34\u6027\u5efa\u7b51\u6d82\u6599\u4e2d\u5206\u522b\u6dfb\u52a0\u7ea61 000\u00d710-6\u76844 \u79cd\u9632\u9709\u5242\u6d3b\u6027\u6210\u4efd\uff0c \u7136\u540e\u7528\u5236\u819c\u5668\u5236\u5907300 \u03bcm\u539a\u5ea6\u7684\u6e7f\u819c\uff0c\u5ba4\u6e29\u667e\u5e72\u3002\u4e4b\u540e\u53d6\u4e00\u90e8\u5206\u8fdb\u884c\u5e72\u819c\u7684UV\u8001\u5316\uff0c\u5728\u7d2b\u5916\u706f\u7bb1\uff08253 nm\uff09\u4e2d\u7d2b\u5916\u7167\u5c04\u4e00\u4e2a\u6708\u3002\u8001\u5316\u7ed3\u675f\u4ee5\u540e\uff0c\u7528\u8272\u5ea6\u4eea\u6d4b\u8bd5\u672a\u8001\u5316\u548c\u8001\u5316\u540e\u5e72\u819c\u7684\u9ec4\u53d8\u60c5\u51b5,\u6d4b3 \u4e2a\u70b9\u53d6\u5e73\u5747\u503c\u3002\u7136\u540e\u5728\u672a\u8001\u5316\u548c\u8001\u5316\u540e\u7684\u5e72\u819c\u4e0a\u5206\u522b\u5747\u5300\u5730\u53d6\u51e0\u5757\uff0c\u7ecf\u8fc7\u6eb6\u5242\u8403\u53d6\u540e\u7528\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6d4b\u8bd5\u5e72\u819c\u4e2d\u9632\u9709\u5242\u7684\u4fdd\u7559\u91cf\u3002\u518d\u53d6\u4e00\u90e8\u5206\u5e72\u819c\u6a21\u62df\u96e8\u6c34\u51b2\u5237\u5899\u9762\u7684\u8fc7\u7a0b\uff1a\u5728\u4e00\u4e2a\u65b9\u6846\u5185\uff0c\u5c06\u6f06\u819c\u60ac\u6302\u6d78\u6ce1\u5728\u81ea\u6765\u6c34\u4e2d\uff0c \u81ea\u6765\u6c34\u4ee5\u6bcf\u5206\u949f800 \u81f31 000 mL\u7684\u6d41\u91cf\u4e0d\u505c\u5730\u4ece\u8fdb\u5165\u7aef\u6d41\u5165\uff0c\u4ece\u51fa\u53e3\u7aef\u6392\u51fa\u3002\u5206\u522b\u51b2\u52371 d \u548c12 d \u540e\u53d6\u51fa\u6f06\u819c\u667e\u5e72\uff0c\u7136\u540e\u901a\u8fc7\u6eb6\u5242\u8403\u53d6\u7684\u65b9\u6cd5\u7528\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6d4b\u8bd5\u521d\u59cb\u53ca\u51b2\u6c34\u540e\u6f06\u819c\u4e2d\u9632\u9709\u5242\u7684\u542b\u91cf\u3002<\/p>\n

2 \u7ed3\u679c\u4e0e\u8ba8\u8bba<\/p>\n

2.1 \u9632\u9709\u6548\u7387\u6d4b\u8bd5<\/p>\n

\u4e3a\u4e86\u6bd4\u8f83\u5404\u6d3b\u6027\u6210\u5206\u5728\u6c34\u6027\u6d82\u6599\u4e2d\u7684\u9632\u9709\u529f\u6548\u53ca\u5e7f\u8c31\u6027\uff0c\u5b9e\u9a8c\u53c2\u7167\u56fd\u6807GB\/T 1741\u20142007 \u8fdb\u884c\u4e86\u9632\u9709\u5b9e\u9a8c\u3002\u8be5\u65b9\u6cd5\u7684\u5177\u4f53\u8bc4\u5206\u6807\u51c6\u89c1\u88682\u3002\u8bc4\u4ef7\u7b49\u7ea7\u901a\u8fc7\u6f06\u819c\u4e0a\u9709\u83cc\u751f\u957f\u7684\u9762\u79ef\u5927\u5c0f\u6765\u8861\u91cf\u3002\u8be5\u7ed3\u679c\u663e\u793a\u6d82\u6599\u54c1\u724c\u7f51<\/a>\uff0c\u6d82\u6599\u4e2dOIT \u6d53\u5ea6\u5728300\u00d710-6\u3001IPBC \u6d53\u5ea6\u5728400\u00d710-6\u3001DCOIT \u6d53\u5ea6\u5728500\u00d710-6 \u65f6\uff0c\u6f06\u819c\u4e0a\u7684\u9709\u83cc\u751f\u957f\u9762\u79ef\u5c0f\u4e8e10%\uff1b\u800cBCM \u6d53\u5ea6\u5728500\u00d710-6 \u65f6\u6f06\u819c\u4e0a\u4ecd\u7136\u6709\u5927\u4e8e10%\u9762\u79ef\u7684\u9709\u83cc\u751f\u957f\u3002\u8868\u660eOIT \u7684\u5e72\u819c\u9632\u9709\u6548\u7387\u6700\u9ad8\uff0c\u800cBCM\u7684\u9632\u9709\u6548\u7387\u76f8\u5bf9\u8f83\u5dee\u3002\u6d4b\u8bd5\u7684\u5177\u4f53\u7ed3\u679c\u89c1\u88683\u3002<\/p>\n

2.jpg<\/p>\n

2.2 \u6297\u83cc\u6d4b\u8bd5<\/p>\n

\u5982\u679c\u73af\u5883\u76f8\u5bf9\u6e7f\u5ea6\u8f83\u4f4e\u7684\u60c5\u51b5\u4e0b\uff0c \u6d82\u6599\u7684\u5e72\u819c\u4e0d\u5177\u5907\u7ec6\u83cc\u751f\u957f\u6240\u9700\u8981\u7684\u6c34\u5206\uff0c \u6240\u4ee5\u5728\u6d82\u6599\u6d82\u88c5\u5230\u5899\u4e0a\u540e\u6211\u4eec\u4e0d\u4e88\u8003\u8651\u5176\u8868\u9762\u6297\u7ec6\u83cc\u7684\u6027\u80fd\u3002\u4f46\u662f\u5728\u4e00\u4e9b\u7279\u6b8a\u7684\u5730\u65b9\uff0c\u6bd4\u5982\u516c\u5171\u573a\u5408\u6216\u8005\u533b\u9662\u7b49\u5730\u65b9\u6211\u4eec\u4f1a\u5e0c\u671b\u6d82\u5c42\u5177\u6709\u4e00\u5b9a\u7684\u6297\u83cc\u6027\u80fd\u3002\u4e3a\u4e86\u6d4b\u8bd5\u5404\u6d3b\u6027\u7269\u8d28\u5728\u6d82\u5c42\u4e2d\u7684\u6297\u83cc\u6027\u80fd\uff0c\u6211\u4eec\u6839\u636eJISZ2801 \u7684\u65b9\u6cd5\u8fdb\u884c\u4e86\u6297\u83cc\u6d4b\u8bd5\u3002<\/p>\n

\u6297\u83cc\u6d3b\u6027=l g \uff0824 h \u7a7a\u767d\u6d82\u6599\u6837\u54c1\u83cc\u6b8b\u7559\u91cf\/24 h\u6dfb\u52a0\u9632\u9709\u5242\u6837\u54c1\u83cc\u6b8b\u7559\u91cf\uff09\uff1b<\/p>\n

\u6740\u83cc\u7387=\uff0824 h \u7a7a\u767d\u6d82\u6599\u6837\u54c1\u83cc\u6b8b\u7559\u91cf-24 h \u6dfb\u52a0\u9632\u9709\u5242\u6837\u54c1\u83cc\u6b8b\u7559\u91cf\uff09\/24 h \u7a7a\u767d\u6d82\u6599\u6837\u54c1\u83cc\u6b8b\u7559\u91cf\uff1b<\/p>\n

\u5728\u8be5\u65b9\u6cd5\u4e2d\uff0c\u6297\u83cc\u6d3b\u6027\u503c\uff1e2\uff0c\u5373\u6740\u83cc\u7387>99%\u5c31\u8868\u660e\u6709\u6709\u6548\u7684\u8868\u9762\u6297\u83cc\u529f\u6548\uff0c\u5177\u4f53\u5b9e\u9a8c\u7ed3\u679c\u89c1\u88684\u3002<\/p>\n

3.jpg<\/p>\n

\u7ed3\u679c\u8868\u660e\uff1a\u5728\u76f8\u540c\u7684500\u00d710-6 \u6d3b\u6027\u7269\u6dfb\u52a0\u91cf\u4e0b\uff0c\u542b\u6709DCOIT \u7684\u6d82\u6599\u8fbe\u5230\u4e8699%\u7684\u6740\u83cc\u7387\uff0c\u800c\u542b\u6709\u6d3b\u6027\u7269OIT\u3001IPBC\u3001BCM \u7684\u6d82\u6599\u6ca1\u6709\u8fbe\u5230\u3002\u8bf4\u660eDCOIT \u5728500\u00d710-6 \u65f6\u53ef\u4ee5\u4e3a\u8be5\u4f53\u7cfb\u7684\u5e72\u819c\u6d82\u5c42\u63d0\u4f9b\u5f88\u597d\u7684\u8868\u9762\u6297\u83cc\u6027\u80fd\uff0c\u800cOIT\u3001IPBC\u3001BCM \u5728\u8be5\u4f53\u7cfb\u4e0b\u7684\u8868\u9762\u6297\u83cc\u6548\u679c\u4e0d\u660e\u663e\u3002<\/p>\n

2.3 4 \u79cd\u9632\u9709\u5242\u5728\u6d82\u6599\u4e2d\u7684\u70ed\u7a33\u5b9a\u6027\u6d4b\u8bd5<\/p>\n

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4.jpg<\/p>\n

2.4 \u8010\u5019\u6027\u6d4b\u8bd5<\/p>\n

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2.4.1 \u6d82\u6599\u5e72\u819c\u9ad8\u6e29\u548cUV \u8001\u5316\u6027\u80fd\u6d4b\u8bd5<\/p>\n

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5.jpg<\/p>\n

7.jpg<\/p>\n

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6.jpg<\/p>\n

2.4.2 \u6d82\u6599\u6d82\u819c\u8010\u51b2\u6c34\u6027\u80fd\u6d4b\u8bd5<\/p>\n

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8.jpg<\/p>\n

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3 \u7ed3\u8bed<\/p>\n

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